| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB110-3638SS | |||
| Azide and BSA Free | NBP2-80568 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-30436SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-70392 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-37507-0.025ml | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-37563-0.025ml | |||
![Immunocytochemistry/Immunofluorescence: Alkaline Phosphatase/ALPP Antibody (8B6) [NB110-3638] - Representative montage images are shown for control dissociated DRG culture. (B) Dissociated DRGs cultures exposed to conditioned medium from alkaline phosphatase-expressing rat GRPs. Image collected and cropped by CiteAb from the following publication (eneuro.org/content/4/1/ENEURO.0171-16.2017), licensed under a CC-BY license.](http://images.novusbio.com/fullsize/Alkaline-Phosphatase-ALPP-Antibody-8B6-Immunocytochemistry-Immunofluorescence-NB110-3638-img0005.jpg "Immunocytochemistry/Immunofluorescence: Alkaline Phosphatase/ALPP Antibody (8B6) [NB110-3638] - Representative montage images are shown for control dissociated DRG culture. (B) Dissociated DRGs cultures exposed to conditioned medium from alkaline phosphatase-expressing rat GRPs. Image collected and cropped by CiteAb from the following publication (eneuro.org/content/4/1/ENEURO.0171-16.2017), licensed under a CC-BY license.")
![Immunocytochemistry/Immunofluorescence: Alkaline Phosphatase/ALPP Antibody (8B6) [NB110-3638] - analysis of ALPP in MDA-MB-231 cells using an anti-ALPP antibody (blue - cell membrane, green - ALPP). Image from verified customer review.](http://images.novusbio.com/fullsize/Alkaline-Phosphatase-ALPP-Antibody-8B6-Immunocytochemistry-Immunofluorescence-NB110-3638-img0003.jpg)
![Western Blot: Alkaline Phosphatase/ALPP Antibody (8B6) [NB110-3638] - Analysis of Alkaline Phosphatase (Placental) expression in JAR whole cell lysate.](http://images.novusbio.com/fullsize/Alkaline-Phosphatase-ALPP-Antibody-8B6-Western-Blot-NB110-3638-img0002.jpg)
![Immunohistochemistry-Paraffin: Alkaline Phosphatase/ALPP Antibody (8B6) [NB110-3638] - Alkaline Phosphatase, Placental Antibody (8B6) [NB110-3638] - IHC staining of Alkaline Phosphatase (Placental) in human placenta using DAB with hematoxylin counterstain. Proteolytic Induced Epitope Retrieval (PIER) was used.](http://images.novusbio.com/fullsize/Alkaline-Phosphatase-ALPP-Antibody-8B6-Immunohistochemistry-Paraffin-NB110-3638-img0001.jpg)
![Flow Cytometry: Alkaline Phosphatase/ALPP Antibody (8B6) [NB110-3638] - Detection of Alkaline Phosphatase/ALPP in Human HeLa Cell Line by Flow Cytometry. Human HeLa cell line was stained with Mouse Anti- Alkaline Phosphatase/ALPP Monoclonal Antibody (Catalog # NB110-3638, filled histogram), or Mouse IgG2A isotype control (Catalog # MAB003, open histogram) followed by APC-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0101B). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005).Images may not be copied, printed or otherwise disseminated without express written permission of Novus Biologicals a bio-techne brand.](http://images.novusbio.com/fullsize/Alkaline-Phosphatase-ALPP-Antibody-8B6-Flow-Cytometry-NB110-3638-img0004.jpg)
![Immunocytochemistry/ Immunofluorescence: Alkaline Phosphatase/ALPP Antibody (8B6) - BSA Free [NB110-3638] - GRPs enhance axonal growth in DRGs in vitro. A–C, Representative montage images are shown for control dissociated DRG culture (A), dissociated DRGs cocultured with rat GRPs (B), & dissociated DRGs cultures exposed to conditioned medium from alkaline phosphatase-expressing rat GRPs (C). beta III-tubulin (red) immunofluorescence highlights the neurons; GRPs are visualized by immunostaining for alkaline phosphatase (green). D shows quantification for the average length of the longest axon per neuron ± SEM (n ≥ 30 neurons in three separate experiments; **p ≤ 0.01 by Student’s t test). Scale bar, 250 μm. Image collected & cropped by CiteAb from the following publication (//www.eneuro.org/lookup/doi/10.1523/ENEURO.0171-16.2017), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb110-3638_mouse-monoclonal-alkaline-phosphatase-alpp-antibody-8b6-310202415371956.jpg)
![Immunocytochemistry/ Immunofluorescence: Alkaline Phosphatase/ALPP Antibody (8B6) - BSA Free [NB110-3638] - GRPs enhance axonal growth in DRGs in vitro. A–C, Representative montage images are shown for control dissociated DRG culture (A), dissociated DRGs cocultured with rat GRPs (B), & dissociated DRGs cultures exposed to conditioned medium from alkaline phosphatase-expressing rat GRPs (C). beta III-tubulin (red) immunofluorescence highlights the neurons; GRPs are visualized by immunostaining for alkaline phosphatase (green). D shows quantification for the average length of the longest axon per neuron ± SEM (n ≥ 30 neurons in three separate experiments; **p ≤ 0.01 by Student’s t test). Scale bar, 250 μm. Image collected & cropped by CiteAb from the following publication (//www.eneuro.org/lookup/doi/10.1523/ENEURO.0171-16.2017), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb110-3638_mouse-monoclonal-alkaline-phosphatase-alpp-antibody-8b6-310202415175250.jpg)
![Immunocytochemistry/ Immunofluorescence: Alkaline Phosphatase/ALPP Antibody (8B6) - BSA Free [NB110-3638] - Coculture with neural & glial progenitor cells increases axonal outgrowth from adult DRG neurons. A–C, Representative montage images of control-dissociated DRG cultures (A), dissociated DRGs cocultured with rat GRPs/NRPs (B), & dissociated DRG cultures exposed to conditioned medium from parallel rat GRP/NRP cultures (C) are shown. Immunofluorescence for beta III-tubulin (red) & nestin (green) highlight DRG neurons & GRP/NRP nuclei, respectively. D, Quantitation of the average lengths of the longest axon per neuron (±SEM) for the above conditions is shown. Coculture with GRPs/NRPs significantly increases in axon length compared with the standard DRG culture; exposure to conditioned medium from GRP/NRP cultures showed a further increase in axon length (n ≥ 30 neurons in three separate experiments; *p ≤ 0.05 & ***p ≤ 0.001 by Student’s t test). Scale bar, 250 μm. E, Quantitation of axon growth parameters for 7 d injury-conditioned DRG neurons cultured on coverslips laid over a bed of GRP/NRP cells (coculture) or control conditions is shown (n ≥ 200 neurons analyzed in three separate experiments; p values represent ANOVA with Tukey post hoc analyses). Image collected & cropped by CiteAb from the following publication (//www.eneuro.org/lookup/doi/10.1523/ENEURO.0171-16.2017), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb110-3638_mouse-monoclonal-alkaline-phosphatase-alpp-antibody-8b6-310202416235514.jpg)
![Immunocytochemistry/ Immunofluorescence: Alkaline Phosphatase/ALPP Antibody (8B6) - BSA Free [NB110-3638] - Coculture with neural & glial progenitor cells increases axonal outgrowth from adult DRG neurons. A–C, Representative montage images of control-dissociated DRG cultures (A), dissociated DRGs cocultured with rat GRPs/NRPs (B), & dissociated DRG cultures exposed to conditioned medium from parallel rat GRP/NRP cultures (C) are shown. Immunofluorescence for beta III-tubulin (red) & nestin (green) highlight DRG neurons & GRP/NRP nuclei, respectively. D, Quantitation of the average lengths of the longest axon per neuron (±SEM) for the above conditions is shown. Coculture with GRPs/NRPs significantly increases in axon length compared with the standard DRG culture; exposure to conditioned medium from GRP/NRP cultures showed a further increase in axon length (n ≥ 30 neurons in three separate experiments; *p ≤ 0.05 & ***p ≤ 0.001 by Student’s t test). Scale bar, 250 μm. E, Quantitation of axon growth parameters for 7 d injury-conditioned DRG neurons cultured on coverslips laid over a bed of GRP/NRP cells (coculture) or control conditions is shown (n ≥ 200 neurons analyzed in three separate experiments; p values represent ANOVA with Tukey post hoc analyses). Image collected & cropped by CiteAb from the following publication (//www.eneuro.org/lookup/doi/10.1523/ENEURO.0171-16.2017), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb110-3638_mouse-monoclonal-alkaline-phosphatase-alpp-antibody-8b6-310202416284919.jpg)
![Immunocytochemistry/ Immunofluorescence: Alkaline Phosphatase/ALPP Antibody (8B6) - BSA Free [NB110-3638] - Coculture with neural & glial progenitor cells increases axonal outgrowth from adult DRG neurons. A–C, Representative montage images of control-dissociated DRG cultures (A), dissociated DRGs cocultured with rat GRPs/NRPs (B), & dissociated DRG cultures exposed to conditioned medium from parallel rat GRP/NRP cultures (C) are shown. Immunofluorescence for beta III-tubulin (red) & nestin (green) highlight DRG neurons & GRP/NRP nuclei, respectively. D, Quantitation of the average lengths of the longest axon per neuron (±SEM) for the above conditions is shown. Coculture with GRPs/NRPs significantly increases in axon length compared with the standard DRG culture; exposure to conditioned medium from GRP/NRP cultures showed a further increase in axon length (n ≥ 30 neurons in three separate experiments; *p ≤ 0.05 & ***p ≤ 0.001 by Student’s t test). Scale bar, 250 μm. E, Quantitation of axon growth parameters for 7 d injury-conditioned DRG neurons cultured on coverslips laid over a bed of GRP/NRP cells (coculture) or control conditions is shown (n ≥ 200 neurons analyzed in three separate experiments; p values represent ANOVA with Tukey post hoc analyses). Image collected & cropped by CiteAb from the following publication (//www.eneuro.org/lookup/doi/10.1523/ENEURO.0171-16.2017), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb110-3638_mouse-monoclonal-alkaline-phosphatase-alpp-antibody-8b6-31020241628494.jpg)
![Immunocytochemistry/Immunofluorescence: TMEM97 Antibody [NBP1-30436] - HeLa cells were fixed in 4% paraformaldehyde for 10 minutes and permeabilized in 0.05% Triton X-100 in PBS for 5 minutes. The cells were incubated with anti-TMEM97 Antibody NBP1-30436 at 1 ug/ml overnight at 4C and detected with an anti-rabbit Dylight 488 (Green) at a 1:1000 dilution for 60 minutes. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 100X objective and digitally deconvolved.](http://images.novusbio.com/fullsize/TMEM97-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-30436-img0008.jpg "Immunocytochemistry/Immunofluorescence: TMEM97 Antibody [NBP1-30436] - HeLa cells were fixed in 4% paraformaldehyde for 10 minutes and permeabilized in 0.05% Triton X-100 in PBS for 5 minutes. The cells were incubated with anti-TMEM97 Antibody NBP1-30436 at 1 ug/ml overnight at 4C and detected with an anti-rabbit Dylight 488 (Green) at a 1:1000 dilution for 60 minutes. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 100X objective and digitally deconvolved.")
![<b>Biological Strategies Validation. </b>Western Blot: TMEM97 Antibody [NBP1-30436] - Expression of proteins involved in extracellular lipid uptake and intracellular cholesterol trafficking following JQ1 (0.4 uM) administration to HepG2 cells for 48 hours. Representative Western blots and densitometric analysis of TMEM97. Tubulin was chosen as loading control. n = 6 independent experiments. Data represent means +/- SD. Statistical analysis was performed by using unpaired Student's t test. ** p < 0.01; *** p < 0.001. Image collected and cropped by CiteAb from the following publication (//www.mdpi.com/1422-0067/21/4/1297), licensed under a CC-BY license.](http://images.novusbio.com/fullsize/TMEM97-Antibody-Western-Blot-NBP1-30436-img0007.jpg)
![Immunohistochemistry-Paraffin: TMEM97 Antibody [NBP1-30436] - Analysis of a FFPE tissue section of human pancreas using 1:200 dilution of TMEM97 antibody. The staining was developed using HRP labeled anti-rabbit secondary antibody and DAB reagent, and nuclei of cells were counter-stained with hematoxylin.](http://images.novusbio.com/fullsize/TMEM97-Antibody-Immunohistochemistry-Paraffin-NBP1-30436-img0009.jpg)
![Western Blot: TMEM97 Antibody [NBP1-30436] - Analysis of TMEM97 in HeLa whole cell extracts.](http://images.novusbio.com/fullsize/TMEM97-Antibody-Western-Blot-NBP1-30436-img0006.jpg)
![Western Blot: Cdk2 Antibody (2D9) [NBP2-00776] Analysis of extracts (35ug) from 9 different cell lines by using anti-Cdk2 monoclonal antibody.](http://images.novusbio.com/fullsize/Cdk2-Antibody-2D9-Western-Blot-NBP2-00776-img0008.jpg "Western Blot: Cdk2 Antibody (2D9) [NBP2-00776] Analysis of extracts (35ug) from 9 different cell lines by using anti-Cdk2 monoclonal antibody.")
![Immunocytochemistry/Immunofluorescence: Cdk2 Antibody (2D9) [NBP2-00776] - Staining of COS7 cells transiently transfected by pCMV6-ENTRY Cdk2.](http://images.novusbio.com/fullsize/Cdk2-Antibody-2D9-Immunocytochemistry-Immunofluorescence-NBP2-00776-img0007.jpg)
![Immunohistochemistry-Paraffin: Cdk2 Antibody (2D9) [NBP2-00776] - Staining of paraffin-embedded Carcinoma of Human lung tissue using anti-Cdk2 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/Cdk2-Antibody-2D9-Immunohistochemistry-Paraffin-NBP2-00776-img0006.jpg)
![Flow Cytometry: Cdk2 Antibody (2D9) [NBP2-00776] - HEK293T cells transfected with either overexpression plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-Cdk2 antibody, and then analyzed by flow cytometry.](http://images.novusbio.com/fullsize/Cdk2-Antibody-2D9-Flow-Cytometry-NBP2-00776-img0003.jpg)
![Western Blot: Cdk2 Antibody (2D9) [NBP2-00776] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY Cdk2 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-Cdk2.](http://images.novusbio.com/fullsize/Cdk2-Antibody-2D9-Western-Blot-NBP2-00776-img0004.jpg)
![Immunocytochemistry/Immunofluorescence: Cdk2 Antibody (2D9) [NBP2-00776] - Immunofluorescent staining of HeLa cells using anti-Cdk2 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/Cdk2-Antibody-2D9-Immunocytochemistry-Immunofluorescence-NBP2-00776-img0005.jpg)
![Flow Cytometry: Cdk2 Antibody (2D9) [NBP2-00776] - Analysis of Hela cells, using anti-Cdk2 antibody, (Red), compared to a nonspecific negative control antibody (Blue).](http://images.novusbio.com/fullsize/Cdk2-Antibody-2D9-Flow-Cytometry-NBP2-00776-img0001.jpg)
![Flow Cytometry: Cdk2 Antibody (2D9) [NBP2-00776] - Analysis of Jurkat cells, using anti-Cdk2 antibody, (Red), compared to a nonspecific negative control antibody (Blue).](http://images.novusbio.com/fullsize/Cdk2-Antibody-2D9-Flow-Cytometry-NBP2-00776-img0002.jpg)
![Western Blot: CDK2 Antibody (1A6) [NBP2-37507] - Analysis using CDK2 mouse mAb against Jurkat (1), HL-60 (2), K562(3), A431(4), HeLa(5), and NIH3T3 (6) cell lysate.](http://images.novusbio.com/fullsize/CDK2-Antibody-1A6-Western-Blot-NBP2-37507-img0003.jpg "Western Blot: CDK2 Antibody (1A6) [NBP2-37507] - Analysis using CDK2 mouse mAb against Jurkat (1), HL-60 (2), K562(3), A431(4), HeLa(5), and NIH3T3 (6) cell lysate.")
![Immunocytochemistry/Immunofluorescence: CDK2 Antibody (1A6) [NBP2-37507] - Analysis of HeLa cells using CDK2 mouse mAb (green). Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.](http://images.novusbio.com/fullsize/CDK2-Antibody-1A6-Immunocytochemistry-Immunofluorescence-NBP2-37507-img0004.jpg)
![Immunohistochemistry-Paraffin: CDK2 Antibody (1A6) [NBP2-37507] - Analysis of colon cancer tissues using CDK2 mouse mAb with DAB staining.](http://images.novusbio.com/fullsize/CDK2-Antibody-1A6-Immunohistochemistry-NBP2-37507-img0007.jpg)
![Flow Cytometry: CDK2 Antibody (1A6) [NBP2-37507] - Analysis of Jurkat cells using CDK2 mouse mAb (green) and negative control (red).](http://images.novusbio.com/fullsize/CDK2-Antibody-1A6-Flow-Cytometry-NBP2-37507-img0005.jpg)
![Western Blot: CDK2 Antibody (1A6) [NBP2-37507] - Analysis using CDK2 mAb against human CDK2 (AA: 197-295) recombinant protein. (Expected MW is 36.8 kDa)](http://images.novusbio.com/fullsize/CDK2-Antibody-1A6-Western-Blot-NBP2-37507-img0002.jpg)
![Immunohistochemistry-Paraffin: CDK2 Antibody (1A6) [NBP2-37507] - Analysis of cervical cancer tissues using CDK2 mouse mAb with DAB staining.](http://images.novusbio.com/fullsize/CDK2-Antibody-1A6-Immunohistochemistry-NBP2-37507-img0006.jpg)
![Immunohistochemistry-Paraffin: CDK2 Antibody (1A6) [NBP2-37507] - Analysis of CDK2 in colon cancer tissue using anti-CDK2 antibody. Image submitted by a verified customer review.](http://images.novusbio.com/fullsize/CDK2-Antibody-1A6-Immunohistochemistry-Paraffin-NBP2-37507-img0008.jpg)
![ELISA: CDK2 Antibody (1A6) [NBP2-37507] - Red: Control Antigen (100ng); Purple: Antigen (10ng); Green: Antigen (50ng); Blue: Antigen (100ng);](http://images.novusbio.com/fullsize/CDK2-Antibody-1A6-ELISA-NBP2-37507-img0001.jpg)
![Western Blot: Cdk6 Antibody (8H4) [H00001021-M01] - Analysis of CDK6 expression in transfected 293T cell line by CDK6 monoclonal antibody (M01), clone 8H4.Lane 1: CDK6 transfected lysate(36.9 KDa).Lane 2: Non-transfected lysate.](http://images.novusbio.com/fullsize/Cdk6-Antibody-8H4-Western-Blot-H00001021-M01-img0015.jpg "Western Blot: Cdk6 Antibody (8H4) [H00001021-M01] - Analysis of CDK6 expression in transfected 293T cell line by CDK6 monoclonal antibody (M01), clone 8H4.Lane 1: CDK6 transfected lysate(36.9 KDa).Lane 2: Non-transfected lysate.")
![Immunocytochemistry/Immunofluorescence: Cdk6 Antibody (8H4) [H00001021-M01] - Analysis of monoclonal antibody to CDK6 on HeLa cell. Antibody concentration 10 ug/ml.](http://images.novusbio.com/fullsize/Cdk6-Antibody-8H4-Immunocytochemistry-Immunofluorescence-H00001021-M01-img0009.jpg)
![Immunohistochemistry-Paraffin: Cdk6 Antibody (8H4) [H00001021-M01] - Analysis of monoclonal antibody to CDK6 on formalin-fixed paraffin-embedded human colon. Antibody concentration 3 ug/ml.](http://images.novusbio.com/fullsize/Cdk6-Antibody-8H4-Immunohistochemistry-Paraffin-H00001021-M01-img0011.jpg)
![Western Blot: Cdk6 Antibody (8H4) [H00001021-M01] - CDK6 monoclonal antibody (M01), clone 8H4. Analysis of CDK6 expression in PC-12.](http://images.novusbio.com/fullsize/Cdk6-Antibody-8H4-Western-Blot-H00001021-M01-img0013.jpg)
![Western Blot: Cdk6 Antibody (8H4) [H00001021-M01] - CDK6 monoclonal antibody (M01), clone 8H4 Analysis of CDK6 expression in Jurkat.](http://images.novusbio.com/fullsize/Cdk6-Antibody-8H4-Western-Blot-H00001021-M01-img0014.jpg)
![Proximity Ligation Assay: Cdk6 Antibody (8H4) [H00001021-M01] - Analysis of protein-protein interactions between CDK2 and CDK6. HeLa cells were stained with anti-CDK2 rabbit purified polyclonal 1:1200 and anti-CDK6 mouse monoclonal antibody 1:50. Each red dot represents the detection of protein-protein interaction complex, and nuclei were counterstained with DAPI (blue).](http://images.novusbio.com/fullsize/Cdk6-Antibody-8H4-Proximity-Ligation-Assay-H00001021-M01-img0007.jpg)
![ELISA: Cdk6 Antibody (8H4) [H00001021-M01] - Detection limit for recombinant GST tagged CDK6 is 0.3 ng/ml as a capture antibody.](http://images.novusbio.com/fullsize/Cdk6-Antibody-8H4-ELISA-H00001021-M01-img0016.jpg)
![Immunohistochemistry-Paraffin: Cdk6 Antibody [NBP1-87262] - Analysis in human placenta and skeletal muscle tissues. Corresponding CDK6 RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/Cdk6-Antibody-Immunohistochemistry-Paraffin-NBP1-87262-img0023.jpg "<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: Cdk6 Antibody [NBP1-87262] - Analysis in human placenta and skeletal muscle tissues. Corresponding CDK6 RNA-seq data are presented for the same tissues.")
![<b>Genetic Strategies Validation and Orthogonal Strategies Validation.</b>Western Blot: Cdk6 Antibody [NBP1-87262] - Analysis in Rh30 cells transfected with control siRNA, target specific siRNA probe #1 and #2. Remaining relative intensity is presented. Loading control: Anti-PPIB.](http://images.novusbio.com/fullsize/Cdk6-Antibody-Western-Blot-NBP1-87262-img0021.jpg)
![Immunocytochemistry/Immunofluorescence: Cdk6 Antibody [NBP1-87262] - Staining of human cell line U-251 MG shows localization to nucleoplasm and cytosol. Antibody staining is shown in green.](http://images.novusbio.com/fullsize/Cdk6-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-87262-img0019.jpg)
![Immunohistochemistry-Paraffin: Cdk6 Antibody [NBP1-87262] - Staining of human skeletal muscle shows no positivity in myocytes as expected.](http://images.novusbio.com/fullsize/Cdk6-Antibody-Immunohistochemistry-Paraffin-NBP1-87262-img0027.jpg)
![<b>Orthogonal Strategies Validation. </b>Western Blot: Cdk6 Antibody [NBP1-87262] - Analysis in human cell lines U2OS and A-549. Corresponding RNA-seq data are presented for the same cell lines. Loading control: Anti-COX4I1.](http://images.novusbio.com/fullsize/Cdk6-Antibody-Western-Blot-NBP1-87262-img0020.jpg)
![Immunohistochemistry-Paraffin: Cdk6 Antibody [NBP1-87262] - Staining of human lymph node shows moderate to strong cytoplasmic positivity in germinal center cells.](http://images.novusbio.com/fullsize/Cdk6-Antibody-Immunohistochemistry-Paraffin-NBP1-87262-img0024.jpg)
![Immunohistochemistry-Paraffin: Cdk6 Antibody [NBP1-87262] - Staining of human testis shows moderate nuclear positivity in cells in seminiferous ducts.](http://images.novusbio.com/fullsize/Cdk6-Antibody-Immunohistochemistry-Paraffin-NBP1-87262-img0025.jpg)
![Immunohistochemistry-Paraffin: Cdk6 Antibody [NBP1-87262] - Staining of human placenta shows strong nuclear positivity in trophoblastic cells.](http://images.novusbio.com/fullsize/Cdk6-Antibody-Immunohistochemistry-Paraffin-NBP1-87262-img0026.jpg)
![Western Blot: Prohibitin Antibody (5H7) [NBP2-37563] - Western blot analysis using PHB mouse mAb against A431 (1), MCF-7 (2), Jurkat (3), Hela (4), HepG2 (5), A549 (6), NIH/3T3 (7), Cos7 (8) and PC-12 (9) cell lysate.](http://images.novusbio.com/fullsize/Prohibitin-Antibody-5H7-Western-Blot-NBP2-37563-img0003.jpg "Western Blot: Prohibitin Antibody (5H7) [NBP2-37563] - Western blot analysis using PHB mouse mAb against A431 (1), MCF-7 (2), Jurkat (3), Hela (4), HepG2 (5), A549 (6), NIH/3T3 (7), Cos7 (8) and PC-12 (9) cell lysate.")
![Immunocytochemistry/Immunofluorescence: Prohibitin Antibody (5H7) [NBP2-37563] - Immunofluorescence analysis of NIH/3T3 cells using PHB mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.](http://images.novusbio.com/fullsize/Prohibitin-Antibody-5H7-Immunocytochemistry-Immunofluorescence-NBP2-37563-img0004.jpg)
![Immunohistochemistry-Paraffin: Prohibitin Antibody (5H7) [NBP2-37563] - Immunohistochemical analysis of paraffin-embedded lung cancer tissues using PHB mouse mAb with DAB staining.](http://images.novusbio.com/fullsize/Prohibitin-Antibody-5H7-Immunohistochemistry-Paraffin-NBP2-37563-img0013.jpg)
![Flow Cytometry: Prohibitin Antibody (5H7) [NBP2-37563] - Flow cytometric analysis of MCF-7 cells using PHB mouse mAb (blue) and negative control (red).](http://images.novusbio.com/fullsize/Prohibitin-Antibody-5H7-Flow-Cytometry-NBP2-37563-img0005.jpg)
![Western Blot: Prohibitin Antibody (5H7) [NBP2-37563] - Western blot analysis using PHB mAb against human PHB (AA: 68-259) recombinant protein. (Expected MW is 46.7 kDa)](http://images.novusbio.com/fullsize/Prohibitin-Antibody-5H7-Western-Blot-NBP2-37563-img0002.jpg)
![Immunohistochemistry-Paraffin: Prohibitin Antibody (5H7) [NBP2-37563] - Immunohistochemical analysis of paraffin-embedded stomach cancer tissues using PHB mouse mAb with DAB staining.](http://images.novusbio.com/fullsize/Prohibitin-Antibody-5H7-Immunohistochemistry-Paraffin-NBP2-37563-img0010.jpg)
![Immunohistochemistry-Paraffin: Prohibitin Antibody (5H7) [NBP2-37563] - Immunohistochemical analysis of paraffin-embedded rectum cancer tissues using PHB mouse mAb with DAB staining.](http://images.novusbio.com/fullsize/Prohibitin-Antibody-5H7-Immunohistochemistry-Paraffin-NBP2-37563-img0011.jpg)
![Immunohistochemistry-Paraffin: Prohibitin Antibody (5H7) [NBP2-37563] - Immunohistochemical analysis of paraffin-embedded liver cancer tissues using PHB mouse mAb with DAB staining.](http://images.novusbio.com/fullsize/Prohibitin-Antibody-5H7-Immunohistochemistry-Paraffin-NBP2-37563-img0012.jpg)
![ELISA: Prohibitin Antibody (5H7) [NBP2-37563] - Red: Control Antigen (100ng); Purple: Antigen (10ng); Green: Antigen (50ng); Blue: Antigen (100ng);](http://images.novusbio.com/fullsize/Prohibitin-Antibody-5H7-ELISA-NBP2-37563-img0001.jpg)
![Western Blot: CDK2 Antibody (OTI2D9) - Azide and BSA Free [NBP2-70392] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY Cdk2 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-Cdk2.](http://images.novusbio.com/fullsize/CDK2-Antibody-OTI2D9-Azide-and-BSA-Free-Western-Blot-NBP2-70392-img0002.jpg "Western Blot: CDK2 Antibody (OTI2D9) - Azide and BSA Free [NBP2-70392] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY Cdk2 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-Cdk2.")
![Immunocytochemistry/Immunofluorescence: CDK2 Antibody (OTI2D9) - Azide and BSA Free [NBP2-70392] - Staining of COS7 cells transiently transfected by pCMV6-ENTRY Cdk2.](http://images.novusbio.com/fullsize/CDK2-Antibody-OTI2D9-Azide-and-BSA-Free-Immunocytochemistry-Immunofluorescence-NBP2-70392-img0007.jpg)
![Immunohistochemistry: CDK2 Antibody (OTI2D9) - Azide and BSA Free [NBP2-70392] - Staining of paraffin-embedded Carcinoma of Human lung tissue using anti-Cdk2 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/CDK2-Antibody-OTI2D9-Azide-and-BSA-Free-Immunohistochemistry-NBP2-70392-img0008.jpg)
![Flow Cytometry: CDK2 Antibody (OTI2D9) - Azide and BSA Free [NBP2-70392] - HEK293T cells transfected with either overexpression plasmid (Red) or empty vector control plasmid (Blue) were immunostaining by anti-Cdk2 antibody, and then analyzed by flow cytometry.](http://images.novusbio.com/fullsize/CDK2-Antibody-OTI2D9-Azide-and-BSA-Free-Flow-Cytometry-NBP2-70392-img0005.jpg)
![Western Blot: CDK2 Antibody (OTI2D9) - Azide and BSA Free [NBP2-70392] - Analysis of extracts (35ug) from 9 different cell lines by using anti-Cdk2 monoclonal antibody.](http://images.novusbio.com/fullsize/CDK2-Antibody-OTI2D9-Azide-and-BSA-Free-Western-Blot-NBP2-70392-img0001.jpg)
![Immunocytochemistry/Immunofluorescence: CDK2 Antibody (OTI2D9) - Azide and BSA Free [NBP2-70392] - Staining of HeLa cells using anti-Cdk2 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/CDK2-Antibody-OTI2D9-Azide-and-BSA-Free-Immunocytochemistry-Immunofluorescence-NBP2-70392-img0006.jpg)
![Flow Cytometry: CDK2 Antibody (OTI2D9) - Azide and BSA Free [NBP2-70392] - Analysis of Hela cells, using anti-Cdk2 antibody, (Red), compared to a nonspecific negative control antibody (Blue).](http://images.novusbio.com/fullsize/CDK2-Antibody-OTI2D9-Azide-and-BSA-Free-Flow-Cytometry-NBP2-70392-img0003.jpg)
![Flow Cytometry: CDK2 Antibody (OTI2D9) - Azide and BSA Free [NBP2-70392] - Analysis of Jurkat cells, using anti-Cdk2 antibody, (Red), compared to a nonspecific negative control antibody (Blue).](http://images.novusbio.com/fullsize/CDK2-Antibody-OTI2D9-Azide-and-BSA-Free-Flow-Cytometry-NBP2-70392-img0004.jpg)
![Western Blot: Prohibitin Antibody (3F4-2B2) [H00005245-M01] - PHB monoclonal antibody (M01), clone 3F4-2B2. Analysis of PHB expression in NIH/3T3.](http://images.novusbio.com/fullsize/Prohibitin-Antibody-3F4-2B2-Western-Blot-H00005245-M01-img0015.jpg "Western Blot: Prohibitin Antibody (3F4-2B2) [H00005245-M01] - PHB monoclonal antibody (M01), clone 3F4-2B2. Analysis of PHB expression in NIH/3T3.")
![Immunocytochemistry/Immunofluorescence: Prohibitin Antibody (3F4-2B2) [H00005245-M01] - Analysis of monoclonal antibody to PHB on HeLa cell. Antibody concentration 1 ~ 10 ug/ml.](http://images.novusbio.com/fullsize/Prohibitin-Antibody-3F4-2B2-Immunocytochemistry-Immunofluorescence-H00005245-M01-img0007.jpg)
![Immunohistochemistry-Paraffin: Prohibitin Antibody (3F4-2B2) [H00005245-M01] - Analysis of monoclonal antibody to PHB on formalin-fixed paraffin-embedded human tonsil tissue.Antibody concentration 5 ug/ml.](http://images.novusbio.com/fullsize/Prohibitin-Antibody-3F4-2B2-Immunohistochemistry-Paraffin-H00005245-M01-img0011.jpg)
![Western Blot: Prohibitin Antibody (3F4-2B2) [H00005245-M01] - PHB monoclonal antibody (M01), clone 3F4-2B2 Analysis of PHB expression in Hela.](http://images.novusbio.com/fullsize/Prohibitin-Antibody-3F4-2B2-Western-Blot-H00005245-M01-img0013.jpg)
![Western Blot: Prohibitin Antibody (3F4-2B2) [H00005245-M01] - PHB monoclonal antibody (M01), clone 3F4-2B2. Analysis of PHB expression in PC-12.](http://images.novusbio.com/fullsize/Prohibitin-Antibody-3F4-2B2-Western-Blot-H00005245-M01-img0014.jpg)
![Immunohistochemistry-Paraffin: Prohibitin Antibody (3F4-2B2) [H00005245-M01] - Analysis of monoclonal antibody to PHB on formalin-fixed paraffin-embedded human colon adenocarcinoma. Antibody concentration 5 ug/ml.](http://images.novusbio.com/fullsize/Prohibitin-Antibody-3F4-2B2-Immunohistochemistry-Paraffin-H00005245-M01-img0009.jpg)
![Sandwich ELISA: Prohibitin Antibody (3F4-2B2) [H00005245-M01] - Detection limit for recombinant GST tagged PHB is approximately 0.03ng/ml as a capture antibody.](http://images.novusbio.com/fullsize/Prohibitin-Antibody-3F4-2B2-Sandwich-ELISA-H00005245-M01-img0012.jpg)
![Western Blot: YB1 Antibody (9J7M4) [NBP3-16213] - Analysis of extracts of various cell lines, using YB1 Rabbit mAb (NBP3-16213) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.](http://images.novusbio.com/fullsize/YB1-Antibody-9J7M4-Western-Blot-NBP3-16213-img0008.jpg "Western Blot: YB1 Antibody (9J7M4) [NBP3-16213] - Analysis of extracts of various cell lines, using YB1 Rabbit mAb (NBP3-16213) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.")
![Immunocytochemistry/Immunofluorescence: YB1 Antibody (9J7M4) [NBP3-16213] - Immunofluorescence analysis of U-2 OS cells using YB1 Rabbit mAb (NBP3-16213) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/YB1-Antibody-9J7M4-Immunocytochemistry-Immunofluorescence-NBP3-16213-img0006.jpg)
![Immunohistochemistry-Paraffin: YB1 Antibody (9J7M4) [NBP3-16213] - Mouse brain using YB1 Rabbit mAb (NBP3-16213) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.](http://images.novusbio.com/fullsize/YB1-Antibody-9J7M4-Immunohistochemistry-Paraffin-NBP3-16213-img0003.jpg)
![Chromatin Immunoprecipitation: YB1 Antibody (9J7M4) [NBP3-16213] - Chromatin immunoprecipitation analysis of extracts of MCF7 cells, using YB1 antibody (NBP3-16213) and rabbit IgG.The amount of immunoprecipitated DNA was checked by quantitative PCR. Histogram was constructed by the ratios of the immunoprecipitated DNA to the input.](http://images.novusbio.com/fullsize/YB1-Antibody-9J7M4-Chromatin-Immunoprecipitation-NBP3-16213-img0010.jpg)
![Western Blot: YB1 Antibody (9J7M4) [NBP3-16213] - Analysis of extracts of HeLa cells, using YB1 Rabbit mAb (NBP3-16213) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.](http://images.novusbio.com/fullsize/YB1-Antibody-9J7M4-Western-Blot-NBP3-16213-img0007.jpg)
![Immunocytochemistry/Immunofluorescence: YB1 Antibody (9J7M4) [NBP3-16213] - Immunofluorescence analysis of C6 cells using YB1 Rabbit mAb (NBP3-16213) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/YB1-Antibody-9J7M4-Immunocytochemistry-Immunofluorescence-NBP3-16213-img0004.jpg)
![Immunocytochemistry/Immunofluorescence: YB1 Antibody (9J7M4) [NBP3-16213] - Immunofluorescence analysis of NIH-3T3 cells using YB1 Rabbit mAb (NBP3-16213) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/YB1-Antibody-9J7M4-Immunocytochemistry-Immunofluorescence-NBP3-16213-img0005.jpg)
![Immunohistochemistry-Paraffin: YB1 Antibody (9J7M4) [NBP3-16213] - Rat brain using YB1 Rabbit mAb (NBP3-16213) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.](http://images.novusbio.com/fullsize/YB1-Antibody-9J7M4-Immunohistochemistry-Paraffin-NBP3-16213-img0001.jpg)
![Immunohistochemistry-Paraffin: YB1 Antibody (9J7M4) [NBP3-16213] - Human oophoroma using YB1 Rabbit mAb (NBP3-16213) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.](http://images.novusbio.com/fullsize/YB1-Antibody-9J7M4-Immunohistochemistry-Paraffin-NBP3-16213-img0002.jpg)
![Immunoprecipitation: YB1 Antibody (9J7M4) [NBP3-16213] - Analysis of 300ug extracts of HeLa cells using 3ug YB1 antibody (NBP3-16213). Western blot was performed from the immunoprecipitate using YB1 (NBP3-16213) at a dilition of 1:1000.](http://images.novusbio.com/fullsize/YB1-Antibody-9J7M4-Immunoprecipitation-NBP3-16213-img0009.jpg)
![Western Blot: SM047 Antibody (SM047) [NBP1-97921] - SM047 immunoprecipitates immunoblotted with the SM047 antibody. Lane 1) UWOV1 lysate, Lane 2) ovarian cancer patients serum and Lane 3) pooled normal serum.](http://images.novusbio.com/fullsize/SM047-Antibody-SM047-Western-Blot-NBP1-97921-img0003.jpg "Western Blot: SM047 Antibody (SM047) [NBP1-97921] - SM047 immunoprecipitates immunoblotted with the SM047 antibody. Lane 1) UWOV1 lysate, Lane 2) ovarian cancer patients serum and Lane 3) pooled normal serum.")
![Immunocytochemistry/Immunofluorescence: SM047 Antibody (SM047) [NBP1-97921] - Staining of a cell from an ovarian cancer cell line, labeled with SMO47 antibody. The green fluorescence shows where the antibody has bound to the glycocalyx of the cell.](http://images.novusbio.com/fullsize/SM047-Antibody-SM047-Immunocytochemistry-Immunofluorescence-NBP1-97921-img0005.jpg)
![Immunohistochemistry: SM047 Antibody (SM047) [NBP1-97921] - Immunohistochemical analysis of SM047 in human ovarian cancer using NBP1-97921. Trypsin antigen retrieval was preformed (0.1%, 37C 10 min).](http://images.novusbio.com/fullsize/SM047-Antibody-SM047-Immunohistochemistry-NBP1-97921-img0004.jpg)
![Western Blot: Prohibitin Antibody [NBP2-19944] - A. 30 ug PC-12 whole cell lysate/extract B. 30 ug Rat2 whole cell lysate/extract12 % SDS-PAGE Prohibitin antibody dilution: 1:1000](http://images.novusbio.com/fullsize/Prohibitin-Antibody-Western-Blot-NBP2-19944-img0006.jpg "Western Blot: Prohibitin Antibody [NBP2-19944] - A. 30 ug PC-12 whole cell lysate/extract B. 30 ug Rat2 whole cell lysate/extract12 % SDS-PAGE Prohibitin antibody dilution: 1:1000")
![Immunocytochemistry/Immunofluorescence: Prohibitin Antibody [NBP2-19944] - SKNSH cells were fixed in 2% paraformaldehyde/culture medium at 37 c for 30 min.Green: Prohibitin protein stained by Prohibitin antibody diluted at 1:1000.Blue: Hoechst 33342 staining.](http://images.novusbio.com/fullsize/Prohibitin-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-19944-img0007.jpg)
![Immunohistochemistry-Paraffin: Prohibitin Antibody [NBP2-19944] - Paraffin-embedded mouse liver. Prohibitin antibody dilution: 1:500.](http://images.novusbio.com/fullsize/Prohibitin-Antibody-Immunohistochemistry-Paraffin-NBP2-19944-img0004.jpg)
![Western Blot: Prohibitin Antibody [NBP2-19944] - Sample (30 ug of whole cell lysate) A: IMR32 B: U87-MG 12% SDS PAGE gel, diluted at 1:1000.](http://images.novusbio.com/fullsize/Prohibitin-Antibody-Western-Blot-NBP2-19944-img0001.jpg)
![Western Blot: Prohibitin Antibody [NBP2-19944] - A. 30 ug Neuro2A whole cell lysate/extract B. 30 ug GL261 whole cell lysate/extractC. 30 ug C8D30 whole cell lysate/extract 12 % SDS-PAGE Prohibitin antibody dilution: 1:1000](http://images.novusbio.com/fullsize/Prohibitin-Antibody-Western-Blot-NBP2-19944-img0005.jpg)
![Immunohistochemistry-Paraffin: Prohibitin Antibody [NBP2-19944] - Immunohistochemical analysis of paraffin-embedded HBL435 xenograft, using antibody at 1:500 dilution.](http://images.novusbio.com/fullsize/Prohibitin-Antibody-Immunohistochemistry-Paraffin-NBP2-19944-img0002.jpg)
![Immunohistochemistry-Paraffin: Prohibitin Antibody [NBP2-19944] - Paraffin-embedded mouse intestine. Prohibitin antibody dilution: 1:500.](http://images.novusbio.com/fullsize/Prohibitin-Antibody-Immunohistochemistry-Paraffin-NBP2-19944-img0003.jpg)